Ebv genome strains, which she ould be useful r sources for ebvrelative studies. Ebv gene expression and regulation human herpesviruses. Ebv is a gammaherpesvirus of the lymphocryptovirus lcv genus and is closely related to other lcvs present in old world nonhuman primates, including ebvlike viruses of chimpanzees and rhesus monkeys. The microbiology society is a notforprofit organization. The human nuclear genome is a highly complex arrangement of two sets of 23 chromosomes, or dna molecules. Genome diversity of epsteinbarr virus from multiple tumor. The virus itself is a dna strand that is composed of somewhere around 85 genes. Epstein barr is, like most viruses, extremely tiny.
Ebv is a herpesvirus with a 184kbp long, doublestranded dna genome that encodes 85 genes. Role of plasma ebv dna levels in predicting recurrence of. Novel ebv rnas and rna structures were identified by computational modeling and rnaseq analyses of ebv. Complete genomic sequence of an epsteinbarr virusrelated herpesvirus. Epsteinbarr virus ebv is a doublestranded dna virus that belongs to the gammaherpesvirus subfamily and was discovered in a burkitts lymphoma bl cell. Little is known concerning the roles of rna structure in this important human pathogen. Epsteinbarr virus ebv is a ubiquitous human herpesvirus associated with the development of both lymphoid and epithelial tumours. Our research spans both fields and so we recognize factors that are very obvious for those in one field, but less so for those in the other. Locoregionally advanced nasopharyngeal carcinomas can be cured by the combination of chemotherapy and radiotherapy. Primary infection is often asymptomatic but results in lifelong infection, which is kept in check by the host immune system. To examine the presence of the ebv genome in specimens, eberish was performed using the inform eber probe ventana medical systems, inc. Epsteinbarr virus is a herpesvirus that causes infectious mononucleosis, carcinomas and immunoproliferative disease. Ebv noncoding rna binds nascent rna to drive host pax5 to.
Ebv is an enveloped virus with icosahedral capsids symmetry and the genome takes on a linear form in mature virions and a circular episomal form during the period. Early ebv genomic diversity and convergence towards the b95. The aim of this prospective study was to evaluate the relationship between viral epstein. Its also called the kissing disease because of one way you can spread it to. Prevalence of human papillomavirus hpv and epsteinbarr. Epsteinbarr virus, which is also known as human herpesvirus 4 hhv4 and which belongs to the herpesviridae family, has a doublestranded dna genome 10. When teens or young adults become infected, it can cause infectious mononucleosis, or mono. Early epsteinbarr virus genomic diversity and convergence. Probing its possible chromatin localization by chart revealed eber2s presence at the terminal repeats trs of the latent ebv genome, overlapping previously identified binding sites for the b cell transcription factor pax5. Genome organization an overview sciencedirect topics. In contrast, doublestrand dna viruses can be subdivided into three groups.
This molecular epidemiology study uses ebv genomic microarray ebvchip to simultaneously detect the prevalent rate and viral gene expression patterns in 57 oral squamous cell carcinoma biopsies. Epsteinbarr virus ebv is a human herpesvirus implicated in cancer and autoimmune disorders. To explore the overall organization of the functional ebv genome, we summarized viral gene expression, transcription. This study provides the first comprehensive genomewide survey of rna and rna structure in ebv. Like other herpesviruses, ebv is a dna virus with a toroidshaped protein core that is.
An atlas of the epsteinbarr virus transcriptome and. Eber2 is an abundant nuclear noncoding rna expressed by the epsteinbarr virus ebv. Plasma epsteinbarr virus dna as an archetypal circulating tumour dna marker. Most people are infected by the epsteinbarr virus ebv in early childhood. In fact, the rhesus monkey lcv and ebv share similar sequences and genetic organization, and are both capable of maintaining infection in the oropharynx and in b.
Review article medical progress columbia university. In eastern countries, plasma levels of viral epsteinbarr deoxyribonucleic acid dna are accurate in predicting recurrence, but few data are available in western populations. Request pdf ebv gene expression and regulation epsteinbarr virus ebv is an extremely efficient virus infecting the majority of the worlds adult population. Parvoviruses are the only virus family with a singlestrand dna genome among the animal viruses. As the term implies, large genome dna viruses can also encode many more proteins. The positions of ebv genes and binding sites for ctcf and ebna1 are indicated. Genomewide analysis of wildtype epsteinbarr virus genomes. In some cases, primary infection can result in infectious mononucleosis. After updating the annotation of the genome, 23 open reading frames were chosen for expression in escherichia coli, initially selecting. Table 2 and figure 1 both show the breadth of genes that can be encoded.
It usually causes no symptoms or only a brief, mild illness. Ebv spreads most commonly through bodily fluids, primarily saliva. Epsteinbarr virus ebv infects more than 90% of the adult population worldwide at some point in their lives, usually with no ill e ects 1. Ebv primarily infects b cells via the highaffinity receptor cd21. Structure and organization of the hsv genome arrangement of sequence elements in hsv dna hsv1 virion dna is a linear molecule containing about 155 000 base pairs kieff et al. Differences in family size between species is the wedge that drives the different. Epsteinbarr virusencoded small rna in situ hybridization biopsy specimens were. An overview of genome organization and how we got there. Furthermore, when hostvirus balance is not achieved, the virus can drive potentially. The symptoms of mono are extreme fatigue, fever, sore throat, and swollen lymph nodes. Pdf on feb 2, 2017, nick gilbert and others published genome organization. Epsteinbarr virus and cancer clinical cancer research.
Small modifications of this pipeline can be used to map chip of viral transcription factors to the human genome. Complete genomic sequence of an epsteinbarr virusrelated. To that end, sequencing the ebvpositive cancer cell genome provides a great solution to reveal the ebv integration distribution, the functional impact of viral integration on the host genome and the ebv genome characteristics. Dx ebv assay 96 37021 direct quantitative detection of. Ebv2 transforms b cells less efficiently than ebv1in vitro, and the viability of ebv2 lymphoblastoid cell lines is less than that of. In general, the genome organization is less complex. These proteins are important in regulating viral genes, replication of the viral dna, providing a structural component for the virions and monitoring the host immune response to the. Although oral cavity is located close to the nasal pharynx, the pathogenetic role of epsteinbarr virus ebv in oral cancers is unclear.
Epsteinbarr virus, frequently referred to as ebv, is a member of the herpesvirus family and one of the most common human viruses. Epsteinbarr is the virus that causes mononucleosis. The nuclear lamina in mammals, the genome is contained within the cell nucleus, a doublemembrane organelle that effectively segregates the transcription machinery from the cytoplasm, where protein production occurs. Ebv share similar sequences and genetic organization, and they maintain persistent infection in the oropharynx and in b cells. Epigenetic specifications of host chromosome docking sites. The nuclear envelope ne and its lamina are such structures. Its genome encodes 86 proteins, which provided targets for a structural genomics project. Eukaryote genome organization of the have been dominated by a mixture of whole genome as well as piecemeal genome duplications. Role of viral and host micrornas in immune regulation of. Ebv1 and ebv2 differ in the organization of the genes that code for the ebv nuclear antigen ebna2, ebna3a, ebna3b, and ebna3c.
Dna revealed colinear genome organization with other gammaherpesviruses. Kmer counting was performing on tumor wgs reads aligned to the ebv genome using jellyfish version 2. Complete genome sequences have now been published for ebv baer et al. Epsteinbarr virus biological agents ncbi bookshelf. Detection of ebv infection and gene expression in oral. Thus the genes presently constituting the mammalian lineage likely stem from a combination of whole genomic amplifications and subsequent reductions of a much smaller genome. You might know this disease better by its nickname, mono. Kwok h, wu cw, palser al, kellam p, sham pc, kwong dlw, chiang aks. Epsteinbarr virus and autoimmune diseases national. Lymphoproliferative disorders after hematopoietic stem. Research paper genomewide analysis of epsteinbarr virus. Progress and problems in understanding and managing. Ebv gene expression and regulation request pdf researchgate.
Genome diversity of epsteinbarr virus from multiple tumor types and normal infection. To profile the ebv genome, we developed an efficient pipeline for aligning highthroughput sequencing reads to the viral genome and subtracting out human reads. There were 10 gaps in the k44mi ebv genome assembled, with gap lengths varying from 1 bp to 105 bp. Ebv is a gammaherpesvirus of the lymphocryptovirus lcv genus and is closely related to other lcvs present in old world nonhuman primates, including ebv like viruses of chimpanzees and rhesus monkeys.
Pdf occupancy of chromatin organizers in the epstein. There are various types of dna sequences and chromosomal arrangements, including single. Epsteinbarr virus ebv, also known as human herpesvirus 4, is a member of the herpes virus family. Determination and genomewide analysis of epsteinbarr virus ebv. Here, we report 40 fulllength ebv genome sequences obtained from matched oral wash. Epsteinbarr virus ebv is a gammaherpesvirus that infects a large fraction of the human population. The prognostic significance of ebv dna load and eber. When infection with ebv occurs during adolescence or young adulthood, it causes infectious mononucleosis 35% to 50% of the time. Our current view of genome organization is based largely on, and perhaps is limited by, data derived mainly fromonlytwotypesofapproaches. The genome of epstein barr virus consists of a linear, doublestranded dna molecule that is 184 kilobase pairs in length. For the k4123mi ebv genome assembly, the ebvrelated sequences identified covered 99% of the lei et al. Epsteinbarr virus is known to cause nasopharyngeal carcinoma.
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